Properties

Structure

A skin equivalent can only be useful in answering scientific questions if it is largely similar to the natural in vivo situation. Our Phenion® Full Thickness Skin Model requires a 6-week production time and consists of an epidermis with a thickness of approximately 8 - 10 cell layers. The epidermis shows all natural skin structures:

stratum basale
stratum spinosum
stratum granulosum
stratum corneum

This epidermis is firmly joined to the underlying layer of fibroblasts which form the dermis. Like human skin, the dermis produces various proteins which show natural differentiations: cytokeratin 10, filaggrin, transglutaminase and involucrin. The epidermal-dermal border is characterised by proteins of the basal membrane such as laminin and collagen IV. The de novo synthesis of elastin and fibronectin are demonstrated in the dermal compartment. The proliferative cells of the stratum basale are identified with Ki-67.

Handling

Conventional cell culture laboratory equipment is sufficient in order to use the Phenion® Full Thickness Skin Model as a tool for in vitro tests. Special equipment is not necessary. The special properties of the matrix gives the Phenion® Full Thickness Skin Model an increased stability and therefore allows easy handling. The 1.3 cm² surface is enough to apply cream formulations simply and effectively.

Use

The model is suitable for testing physiological effects after topical treatment or systemic treatment of the medium. The effects can be followed up after at least a 7-day period.