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Immunohistology

Immunofluorescent methods can be utilized to detect a wide variety of proteins in the skin. Skin models are used in order to measure the effect of substances or formulations on the expression of different proteins in the skin. Full thickness skin or dermis models like the Phenion® Full Thickness Skin Model have to be used to detect proteins in the dermis.

The proteins, e.g. laminine, are marked with specific antibodies and shown by means of fluorescently marked second antibodies. The detection of immunofluorescence is semi-quantitative and evaluated using optical methods.

Images 1-3 show the detection of laminine in the basal membrane of a full-skin model.

Soothing the Skin
HET-CAM

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