Primary Human Skin Cells

Post circumcision, juvenile foreskin tissue from male individuals was stored appropriately and processed within 24 h. Cells are defined as in passage 0 (P0) when derived directly from the skin tissue and grown the first time in monolayer cell culture flasks. After confluency, the cells where harvested and propagated in new cell culture flasks to generate passage 1 cells (P1-cells) until harvested and stored above liquid nitrogen, at -196 °C. We provide 1.0 Mio P1-cells (keratinocytes or fibroblast) per cryo-vial. 

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Open Slideshow Microscopic view of fibroplasts.

Morphology of human primary fibroblasts.

Open Slideshow Image of stacked flasks in laboratory.

Cell culture flasks.

Open Slideshow Microscopic view of keratinocytes.

Human primary keratinocytes in monolayer culture format.

Open Slideshow Microscopic view of human feeder cells.

Feeder cells support cultivation of Human Primary Keratinocytes.

For an optimal culture of Phenion keratinocytes we recommend to make use of feeder cells in a ratio of 1:1. Respective feeder cells, generated from dermal fibroblasts at higher passages, are growth-inhibited using Mitomycin C and routinely provided with 1.0 Mio cells per cryo-vial.

Beside other quality criteria, every cell batch is tested for the absence of HIV-1, Mycoplasma, Hepatitis B and C at an independent third party CRO. Respective certificates of analyses (CoA’s) are provided with every cell shipment (see examples). 

Views:

4/27/17

Primary Human Keratinocytes supported by Feeder Cells

The video shows a six days culture period.

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Views:

5/3/17

Primary Human Fibroplasts

The video shows a three days culture period.

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Certificate examples

Preview image of pdf certificate Phenion human dermal Fibroblasts

CoA Phenion human dermal Fibroblasts

CoA Phenion human dermal Fibroblasts

Preview image of pdf with certificate for human epidermal keratinocytes

CoA Phenion human epidermal Keratinocytes

CoA Phenion human epidermal Keratinocytes

Preview image of pdf download of certificate Phenion human feeder cells

CoA Phenion human Feeder Cells

CoA Phenion human Feeder Cells

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