Reconstructed Tissues

The reconstructed tissue models allow the testing of the influence of raw materials and products on cell growth and other properties. 

  • Phenion FT Skin Model
  • Phenion FT AGED Skin Model

The human Phenion Full-Thickness Skin Model mimics native human skin in diverse dimensions, such as histological architecture and a wide spectrum of physiological skin parameters. Its biological equivalence with human skin makes the tissue perfectly suited as alternative to animal testing (e.g. 3D-Skin Comet-assay for genotoxicity testing) and in further research applications, as listed below. 

Fields of application: 

  • Basic skin physiological and biochemical research
  • Basic dermatological/medical research
  • Claim substantiation for cosmetic ingredients
  • Transdermal drug delivery studies
  • Penetration studies
  • Mode of action of pharmaceuticals and actives
  • Wound healing studies
  • Analysis of environmental effects on skin physiology, e.g. UV and IR irradiation
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Phenion FT Skin Model

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Visualization of exemplary differentiation proteins by immunofluorescent labeling.

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Immunohistology shows well organized epidermal differentiation.

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Ki67 antibody localizes proliferating basal keratinocytes in the epidermis.

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Cytokeratin 10/15 immunostaining of matured Phenion FT Skin Model.

Open Slideshow Microscopic view optical coherence tomography

Optical coherence tomography (OCT) is used as non-invasive tool allowing fine-meshed batch control. 

A minimal order quantity for one Phenion FT Skin Model Kit contains six full thickness skin tissues and can be purchased at a price of 750.-€/kit. Please note respective kits with higher skin model quantities can be purchased at a price of 125.-€ per additional tissue, according to customer needs. The Phenion FT Skin Model Kits are equipped with sufficient materials required for the manipulation and culture of the tissues. The materials delivered with the kits enable individual tissue cultivation in six separated vessels or the joint cultivation ‏(e.g. 2 x 3 or 1 x 6) of the skin models.

Content of the Phenion FT Skin Model kit*
6Full Thickness Skin Models (placed on transport-agar in a 24-well plate)
6Sterile filter spacers
6Sterile small petri dishes (35/10 mm)
2Sterile large petri dishes (100/20 mm)
6Sterile pieces of filter paper (approx. 20 x 20 mm)
1Sterile piece of filter paper (approx. 55 x 65 mm)

*Please order 250 ml ALI-Medium (ready-to-use) separately to culture six skin models.

Content of the Phenion FT skin model kit

The Phenion FT Skin model is ideally suited to study epidermal – dermal interactions. The model allows analyses of the complete tissue as well as the compartments dermis and epidermis, separately (for protocols see download center). In these cases, the following protocol guides through the separating process, allowing researchers to individually assess the epidermis and dermis – e.g. for DNA, RNA or protein examinations. 

Preview image of skin model instructions pdf for download.

Phenion FT Skin Model Kit Instructions for Use

For further instructions, please take note of the Phenion FT Skin Model Kit Instructions for Use, available here. 

Phenion FT Skin Model Kit Instructions for Use

Our latest innovation - the Phenion FT AGED Skin Model is a surrogate for aged human skin. It mimics mature human skin, both structurally and physiologically. The Phenion FT AGED Skin Model, is characterized by thinner epidermis and dermal tissues with senescent fibroblasts, reduced collagen and elastin synthesis, and elevated MMP secretion (Scholz, 2014; Dieckmann et al., 2016). With these features, the Phenion FT AGED Skin Model is an ideal research tool to study the basic biology of skin ageing mechanisms. 

Phenion FT skin model compared to Phenion Aged FT skin model

Characteristic of the Phenion® AGED FT Skin Model - the dermal compartment reveals reduced collagen and elastic network.

A minimal order quantity for one Phenion FT AGED Skin Model Kit contains six full thickness skin tissues and can be purchased at a price of 900.-€/kit. Please note respective kits with higher skin model quantities can be purchased at a price of 150.-€ per additional tissue, according to customer needs. The Phenion FT AGED Skin Model Kits are equipped with sufficient materials required for the manipulation and culture of the tissues. The materials delivered with the kits enable individual tissue cultivation in six separated vessels or the joint cultivation ‏(e.g. 2 x 3 or 1 x 6) of the skin models.

Phenion FT SKin Model Fibulin 5

Detection of Fibulin-5 by immunolocalization in freeze sections of the Phenion® AGED FT Skin Model compared to the standard skin model type.

In the Phenion FT AGED Skin Model the dermal fibroblasts are treated with mitomycin C (MMC) for 48 hours before adding the keratinocytes during the production process. The cytostatic drug MMC is an alkylating agent, which, after becoming activated within the cells, leads to covalent crosslinking of the DNA strands (Lee et al., 2006). Crosslinked DNA sections cannot be further transcribed or replicated, and cell proliferation stops. In addition, activated MMC generates reactive oxygen species (ROS), which can damage the DNA, too (Alili et al., 2014). Both effects, cell cycle arrest and ROS generation, are characteristic for the extrinsic and intrinsic ageing of the human skin. It could be demonstrated that human dermal fibroblasts can be arrested in the cell cycle and driven into senescence when they are treated with MMC in culture.

Content of the Phenion FT AGED Skin Model kit*
6Full Thickness Skin Models (placed on transport-agar in a 24-well plate)
6Sterile filter spacers
6Sterile small petri dishes (35/10 mm)
2Sterile large petri dishes (100/20 mm)
6Sterile pieces of filter paper (approx. 20 x 20 mm)
1Sterile piece of filter paper (approx. 55 x 65 mm)

*Please order 250 ml ALI-Medium (ready-to-use) separately to culture six Skin Models.

Preview image of skin model instructions pdf for download.

Phenion FT Skin Model Kit Instructions for Use

For further instructions, please take note of the Phenion FT Skin Model Kit Instructions for Use, available here. 

Phenion FT Skin Model Kit Instructions for Use

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