Do you offer in vitro services?
If you are interested in in vitro test services, please contact us at info@phenion.com.
Which applications can be run with the epiCS models?
The epiCS models are validated for the in vitro skin corrosion (OECD TG 431) and skin irritation test (OECD TG 439). They are also suited for many other applications like the epiCS skin sensitization and potency test, the skin irritation test for medical devices, the phototoxicity test and the micronucleus assay.
Which applications can be run with the different FT skin models?
FT skin models can be used for many different applications, i.e. for genotoxicity assessment (3D skin comet assay), wound healing studies, TEWL measurement, collagen and elastin synthesis, transdermal drug delivery, skin barrier tests, microbiology etc.
Is the skin irritation test using epiCS models regulatory accepted?
Yes. It is part of the OECD Test Guideline 439.
Is the skin corrosion test using epiCS models regulatory accepted?
Yes. It is part of the OECD Test Guideline 431.
How can we validate the in vitro skin irritation and/or corrosion test in our lab?
We are offering epiCS validation kits for both endpoints to run a validation study in parallel at your and in our lab. Successful validation is certified respectively. Please contact us at info@phenion.com.
For which application do I need the nylon meshes and filter discs?
Nylon meshes are used for homogenous distribution of liquid test substances on the skin model surface in the epiCS skin irritation test and the epiCS corrosion test. Filter Discs are needed for epiCS® skin sensitization and potency test.
Is it possible to add bacteria or other microorganisms onto the FT skin models?
For any microbiological study we recommend working with our INSERT FT skin model. It is identical with the “standard” FT skin model but grown in a co-culture insert. This special feature allows the topical application of bacteria suspensions without risking that the microbes might escape into the underlying cell culture medium and consequently jeopardize your test.
Is it possible to inject substances or microorganisms into the skin?
Yes, it is possible to inject substances into the FT skin equivalent. Due to its mechanical robustness, it does not break during the manipulation (Stellavato et al., 2016).
Do you provide any skin model suitable for wound healing?
Wound healing studies can be conducted with the FT skin models.
Different wounding mechanisms can be applied:
- partial epidermal removal
- cutting
- thermal tissue damage.
The progress of wound healing can be monitored non-invasively with optical coherence tomography (OCT), or on the basis of histological sections.
Is the skin model suitable for the study of transdermal drug delivery by patches?
Yes. A patch can be applied onto the near-dry tissue surface, and drug distribution and permeation, respectively, can be analyzed with HPLC, MS etc.
We would like to run the skin sensitization and potency test (SSPT) in our lab for the first time. What are your recommendations?
We offer a SSPT training kit including epiCS models, culture medium, assay medium, MTT reagent, 6-, 24- and 96-well plates, solvents and controls which helps you to easily run the skin sensitization and potency test in your lab. For price information please contact us at info@phenion.com.
Where do I get support for establishing the 3D skin comet assay in my lab?
Please, contact us at info@phenion.com. We support you with our expertise to implement the 3D skin comet assay in your lab.
How do I apply a test substance on top of the tissue?
Liquids can be applied with a pipette, solids with a sharp spoon or spatula. Viscous material can be applied either with a paintbrush or a displacement pipette.
Is it possible to separate epidermis from dermis for severally compartment analysis?
Yes. Incubation of the FT skin model with thermolysin allows the separation of epidermis from dermis with the help of forceps.