Reconstructed Tissues

Our reconstructed skin models have been optimized to analyze the impact of chemicals, active ingredients and products on tissue architecture and physiological properties like cell growth, skin metabolism, the synthesis of collagen, elastin and other extracellular matrix proteins and many more.


  • Phenion FT Skin Model
  • Phenion FT AGED Skin Model

The human Phenion Full-Thickness Skin Model mimics native human skin in diverse dimensions, such as in histological architecture and in a wide spectrum of physiological and biochemical parameters. Its biological equivalence with human skin makes the tissue perfectly suited as in vitro alternative to animal testings, e.g. in the 3D Skin Comet assay for genotoxicity testing and in a vast variety of research applications as listed below. 

Fields of application: 

  • Basic research in skin physiology and biochemistry
  • Clinical dermatological research
  • Claim substantiation for cosmetic ingredients
  • Transdermal drug delivery studies
  • Skin penetration studies
  • Mode of action of pharmaceuticals and actives
  • Wound healing studies
  • Toxicological assessment of chemicals and products
  • Analysis of environmental effects on skin physiology, e.g. UV and IR irradiation

 The Phenion FT Skin model is ideally suited to study the interactions between epidermis and dermis. Based on our special culture conditions, the skin model differentiates and stratifies similar to native human skin, which includes the generation of a horny layer. Analyses can be conducted with the whole tissue as well as with both compartments, dermis and epidermis, separately. A protocol for the enzymatic separation of the tissues can be found in our download center, together with protocols for the extraction and analysis of DNA, RNA and proteins from the skin tissue.

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Phenion FT Skin Model

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Visualization of exemplary differentiation proteins by immunofluorescent labeling.

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Immunohistology shows well organized epidermal differentiation.

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Ki67 antibody localizes proliferating basal keratinocytes in the epidermis.

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Cytokeratin 10/15 immunostaining of matured Phenion FT Skin Model.

Open Slideshow Microscopic view optical coherence tomography

Optical coherence tomography (OCT) is used as non-invasive tool allowing fine-meshed batch control. 

Order Details:

The minimal order quantity comprises 6 FT skin models (Phenion FT Skin Model Kit, order number FT SM-6) and can be purchased at a price of 750.-€/kit. Please note: kits with higher skin model quantities can be purchased at a price of 125.-€ per additional tissue, according to your individual needs. All Phenion FT Skin Model Kits are equipped with sufficient materials required for the culture of the tissues. The materials delivered with the kits enable individual tissue culture in six separate vessels or the joint cultivation ‏(e.g. 2 x 3 or 1 x 6) of the skin models.

Content of the Phenion FT Skin Model kit*
6Full Thickness Skin Models (placed on transport-agar in a 24-well plate)
6Sterile filter spacers
6Sterile small petri dishes (35/10 mm)
2Sterile large petri dishes (100/20 mm)
6Sterile pieces of filter paper (approx. 20 x 20 mm)
1Sterile piece of filter paper (approx. 55 x 65 mm)

*Please order 250 ml ALI-Medium (ready-to-use) separately to culture six skin models.

Content of the Phenion FT skin model kit

Preview image of skin model instructions pdf for download.

Phenion FT Skin Model Kit Instructions for Use

For further instructions, please take note of the Phenion FT Skin Model Kit Instructions for Use, available here. 

Phenion FT Skin Model Kit Instructions for Use

Our latest innovation - the Phenion FT AGED Skin Model - is a surrogate for aged human skin. It mimics mature human skin, both in structure and physiology. The Phenion FT AGED Skin Model is characterized by a thinner epidermis and a connective tissue with senescent fibroblasts, reduced synthesis of extracellular matrix (ECM) proteins like collagen and elastin, and elevated MMP secretion (Scholz, 2014, Master thesis; Dieckmann et al., 2016). With these features, the Phenion FT AGED Skin Model is an ideal research tool to study the basic biology of skin ageing mechanisms and to develop formulations which can delay or even counteract ageing.

Phenion FT SKin Model Fibulin 5

Detection of Fibulin-5 by immunolocalization in freeze sections of the Phenion® AGED FT Skin Model compared to the standard skin model type.

In the Phenion FT AGED Skin Model the dermal fibroblasts are treated with mitomycin C (MMC) for 48 hours before adding the keratinocytes. The cytostatic drug MMC is an alkylating agent, which, after becoming activated within the cells, leads to covalent crosslinking of the DNA strands (Lee et al., 2006). Crosslinked DNA sections cannot be further transcribed or replicated, and cell proliferation stops. In addition, activated MMC generates reactive oxygen species (ROS), which can damage the DNA, too (Alili et al., 2014). Both effects, cell cycle arrest and ROS generation, are characteristic for the extrinsic and intrinsic ageing of the human skin. It could be demonstrated that human dermal fibroblasts can be arrested in the cell cycle and driven into senescence when they are treated with MMC in culture.


Order details:

Phenion FT skin model compared to Phenion Aged FT skin model

Characteristic of the Phenion® AGED FT Skin Model - the dermal compartment reveals reduced collagen and elastic network.

The minimal order quantity comprises 6 FT AGED skin models (Phenion FT AGED Skin Model Kit, order number FT AGED-6) and can be purchased at a price of 900.-€/kit.

Please note: kits with higher skin model quantities can be purchased at a price of 150.-€ per additional tissue, according to your individual needs. The Phenion FT AGED Skin Model Kits are equipped with sufficient materials required for the culture of the tissues. The materials delivered with the kits enable individual tissue culture in six separate vessels or the joint cultivation ‏(e.g. 2 x 3 or 1 x 6) of the skin models.

Content of the Phenion FT AGED Skin Model kit*
6Full Thickness Skin Models (placed on transport-agar in a 24-well plate)
6Sterile filter spacers
6Sterile small petri dishes (35/10 mm)
2Sterile large petri dishes (100/20 mm)
6Sterile pieces of filter paper (approx. 20 x 20 mm)
1Sterile piece of filter paper (approx. 55 x 65 mm)

*Please order 250 ml ALI-Medium (ready-to-use) separately to culture six Skin Models.

Preview image of skin model instructions pdf for download.

Phenion FT Skin Model Kit Instructions for Use

For further instructions, please take note of the Phenion FT Skin Model Kit Instructions for Use, available here. 

Phenion FT Skin Model Kit Instructions for Use

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